Direct detection ofmecA,blaSHV, blaCTX-M,blaTEMandblaOXAgenes from positive blood culture bottles by multiplex-touchdown PCR assay
نویسندگان
چکیده
منابع مشابه
Detection of Candida albicans from Positive Blood Culture Bottles
Objective: To determine the efficacy of direct germ tube method in early detections of candida albicans. Introduction: Blood stream infections (BSIs) caused by Candida species are on the increase and associated with high mortality rates, which are due in part to delay in the administration of appropriate antifungal therapy. Earlier identification of yeast isolates from blood cultures may improv...
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A LightCycler real-time PCR hybridization probe-based assay which detects a partial Klebsiella pneumoniae 16S rRNA gene was developed for the rapid identification of K. pneumoniae directly from growth-positive blood culture bottles (BACTEC 9240 system) within 2 h. No cross-reactivity was observed with 65 negative-control blood cultures that grew bacteria other than K. pneumoniae and 48 negative...
متن کاملSeminested PCR for detection and identification of Candida species directly from blood culture bottles.
We investigated the performance of a seminested PCR (snPCR) assay carried out directly from overnight incubated blood culture bottles of 50 newborn intensive care unit (NICU) patients with suspected candidemia and compared these, for sensitivity, specificity and reliability with results from blood cultures. All positive blood cultures (n = 17) yielded positive results for snPCR, which detected ...
متن کاملA quick and low-cost PCR-based assay for Candida spp. identification in positive blood culture bottles
BACKGROUND Differences in the susceptibility of Candida species to antifungal drugs make identification to the species level important for clinical management of candidemia. Molecular tests are not yet standardized or available in most clinical laboratories, although such tests can reduce the time required for species identification, as compared to the conventional culture-based methods. To dec...
متن کاملEvaluation of combined use of the MALDI-TOF and GenomEra MRSA/SA assay for the direct detection of methicillin resistance in Staphylococcus aureus from positive blood culture bottles.
Months D–F S–D D–F D–F D–F D–F D–F Patients 229 341 314 248 206 117 157 RSV 121 102 99 121 87 68 89 Rhinovirus 64 118 63 56 61 27 44 Human bocavirus 24 41 45 33 16 7 7 Adenovirus 17 28 43 16 17 8 19 Parainfluenza (1, 2, 3, 4) 15 40 7 7 7 4 3 Human metapneumovirus 8 0 6 8 1 1 6 Influenza (A, B, C) 28 38 24 14 14 7 10 Enterovirus 9 7 2 5 7 1 4 Coronavirus 2 6 1 12 2 0 5 Negative 34 69 98 51 49 18 29
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2017
ISSN: 0266-8254
DOI: 10.1111/lam.12676